Preparation and cultivation of rabbit bone marrow-derived mesenchymal stem cells.

In this study,an effective method to prepare and culture rabbit bone marrow derived mesenchymal stem cells(MSCs) was established.After the femurs were dissected from rabbit,the marrow was flushed out with DMEM medium under aseptic condition.The MSCs were enriched and expanded by using bone marrow adherent culture,and inverted microscope was employed to observe the proliferation and their morphous of primary and passage cells.The result shows that MSCs are adherent cells with a similar fibroblastoid spindle-shaped morphology during different passages.Bone marrow adherent culture can be used to produce a certain purity of MSCs,which is an easy way to ensure a good condition and powerful productivity for cell.