In vitro Susceptibility of 191 Clinical Isolated Strains of ESBLs Positive Bacteria

OBJECTIVE To investigate the clinical isolated strains of ESBLs positive bacteria for the rate of occurrence, species distribution and susceptibility to antibiotics, and to look for a practical method to improve the (antibacterial) activity of ceftriaxone to ESBLs positive bacteria. METHODS A total of 574 clinical isolates of Gram-negative bacilli were tested for ESBLs and antimicrobial susceptibility testing was done. Then the (antimicrobial) susceptibility of ceftriaxone added different dose of β-lactamase inhibitor to 191 ESBLs positive strains was evaluated. RESULTS From them 191 clinical isolates of Gram-negtive bacilli produced ESBLs, with a percentage of 33.28%. Detected rate of Acinetobacter was the highest: 43.22%, then Escherichia coli and (Klebsiella.) The resistance rate of ESBLs positive strains to antibiotics was higher than that of ESBLs negative ones. The antibacterial activity of ceftriaxone/clavulanic acid (2∶1) was the best. CONCLUSIONS Though the species distribution of ESBLs positive bacteria is different in different geographycal areas, they are important pathogens to cause nosocomial infection. Rational administration of antibiotics is critical to anti-infection treatment and (nosocomial ) infection control. Routinely detection of ESBLs is necessary.