Study on Rooting and Culture of Spring Vicia faba L.Plantlet

[Objective] The study was to provide the theoretical basis for the seed rapid propagation of Vicia faba L. by using the technique of tissue culture.[Method] With the Qinghai 9 as the tested materials,the rooting effects of the different kinds of growth regulators,BA and the growth regulators,IBA with high concn.and basal part shading on plantlet were studied.After 30 d of treatment,the rooting plants,the root number of each plant and the root length were added up and the rooting rate was calculated.[Result] The rooting rate was 0 when the IAA was used only.The effect of IBA with 1.0 mg/L concn.was the best,its rooting rate was 53.20%,the average root number of each plant was 3.39,and the average root length was 2.84 cm.The rooting rate was 0 when the IAA and the BA was combined with 3 different concn.The effect of the treatment with 0.04 mg/L BA+1.4 mg/L IBA was the best among the combinations of IBA and BA with 3 concn.and the rooting percentage was 73.10%.The effect of IBA with the concn.of 100 mg/L was the best,its rooting percentage was 50.32%,the average number of root was 3.21,and the average root length was 2.26 cm.The rooting effect of the basal part shading was worse than that of darkness culture [Conclusion] The rooting rate of the treatment of 0.04 mg/L BA+1.4 mg/L IBA was the highest.