Inhibiting Mechanism of Coix Lacryma-Jobi Injection Combined with Radiotherapy in Human Lung Carcinoma Cells A549

Objective To investigate the inhibiting mechanism of Coix Lacryma-Jobi Injection(Kanglaite)combined with external irradiation in human lung carcinoma cells A549.Methods Lung carcinoma cells A549 were divided randomly into groups A549 control (given 1640 solution),A549 kanglaite (given 20 μl kanglaite),A549 irradiation ( given 4 Gy X ray ),A549 combination (given 20 μl kanglaite and 4 Gy X ray).Human brain microvascular endothelial cells (HBMEC) were divided randomly into groups HBMEC control (given 0.9% sodium chloride solution),HBMEC kanglaite (given 20 μl kanglaite).The inhibition rates were determined by SRB staining in vitro,and the expressions of proliferating cell nuclear antigen(PCNA),p53,Bcl-2,Bax by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot.Results Kanglaite combined with irradiation had obvious influence on the inhibition rate,the expressions (mRNA and proteins) of PCNA,p53,Bcl-2,Bax (P0.05).Compared with A549 control group,the inhibition rate and the expressions of PCNA,Bcl-2 decreased,the expressions of p53,Bax increased in groups A549 Kanglaite,A549 irradiation,A549 Kanglaite combination (P0.05),and there was no significant difference between groups HBMEC Kanglaite and HBMEC control (P0.05).Conclusion Kanglaite can inhibit the growth of A549 cells by regulating proliferation and apoptosis of tumor cells.Combination of Kanglaite and CDDP can increase chemosensitivity of A549 cells.