Mechanism Research on the Reversion of Multidrug Resistance in Lung Carcinoma Cells A549 Treated by Coix Lacryma-Jobi Injection

Objective To investigate the reversal effect of multidrug resistance(MDR) in lung carcinoma cells treated by Coix Lacryma-Jobi Injection(Kanglaite) and to discuss the mechanism of multidrug resistance (MDR).Methods The lung carcinoma cells A549 was cultured in vitro and treated by Kanglaite in different doses.Inhibition rates of drugs were measured by sulphorhodamine b (SRB) dyeing method.The expressions of MDR1/P-gp,Survivin,B cell lymphoma/lewkmia-2(Bcl-2) and Bcl-2 associated X protein(Bax) were measured by reverse transcription-polymerase chain reaction (RT-PCR),and P-gb,Survivin,Bcl-2 and Bax were measured by Western blot.Results The inhibition rates have significant difference among 4 groups of lung carcinoma cells A549 treated by different concentration of Kanglaite(P0.05).Compared with the control group,the inhibition rate and the expressions of MDR1/P-gp、Survivin、Bcl-2 were decreased,the expressions of Bax were increased in groups of low,medium and high dose of Kanglaite,the difference is significant.The difference between groups of medium and high dose of Kanglaite is also significant(P0.05).Conclusion Kanglaite can inhibit the growth of lung carcinoma cells,and it also can reverse MDR of lung carcinoma cells by regulating the expression of multidrug resistance factors.