Myocardial NF-κB activity in SAP rats and intervention effect of recombinant staphylokinase and Chinese crude drug

[Objective]To investigate myocardial NF-κB mRNA activity in the pathogenesis of severe acute pancreatitis-associated myocardial damage and the intervention effect of the recombinant staphylokinase on it.[Methods]Sixty three male SD rats were divided randomly into the sham-operated group(A group,n=9),the control group(B group,n=27) and Yihuo Qing Yi Decoction plus recombinant staphylokinase(r-SAK) treatment group(r-SAK)(C group,n=27).SAP was induced by intraductal administration of 5% sodium taurocholate.Artery blood of the A,B,C group was obtained at 6h,12h and 24h after induction of the model respectively.Serum concentrations of TNF-α and IL-6 were measured with ELISA.The mRNA expression of NF-κB in myocardium tissue was evaluated by RT-PCR.The myocardium tissue of NF-κB protein was determined by SP immunohistochemical technique.Pancreatic and myocardial tissues were stained with HE and examined under light microscopy.[Results] The myocardial NF-κB expression and the serum levels of TNF-α and IL-6 was significantly increased in 6 hours after induction of SAP.Myocardial NF-κB expression and serum TNF-α,IL-6 in the treatment group were significantly decreased,compared with those of the control group(P0.05).The treatment group of pancreatic and myocardial tissues in pathological changes was alleviated in contrast to the control group.[Conclusion ]The myocardial damage might attribute to myocardial NF-κB activation due to an increase inTNF-α,IL-6 of the blood circulation in SAP rats.Yihuo Qing Yi Decoction plus recombinant staphylokinase(r-SAK) has an beneficial role in the prevention and therapy of SAP associated myocardial damage.