DETECTION OF ATENOLOL IN PLASMA BY SPE COMBINED WITH HPLC AND RELATIVE BIOAVAILABILITY OF ATENOLOL TABLETS

OBJECTIVE:To establish a method to determine atenolol concentration in plasma by SPE HPLC, and to study relative bioavailability of atenolol tablets.METHODS:The atenolol concentrations in plasma through solid phase extraction SFE were determined by HPLC method following a single oral dose were given to 12 volunteers. RESULTS: RSD in a day and between days were less than 10% and recovery rates of plasma coincided for pharmacokinetic study. The pharmacokinetic parameters of xperimental tablets and comparative tablets,AUC 0 36 , C max , T max , t 1/2 were 5 150.35±1 172.52 ng·h·ml 1 and 5 466.50±1 277.08 ng·h·ml 1 ,717.04±189.69 ng·ml 1 and 678.63±193.36 ng·ml 1 , 2.42±0.45 h and 2.63±0.54 h, 7.84±1.25 h and 7.73±1.59 h. There were no significant different between the two formulations in the AUC, T max , C max .CONCLUSION: The SPE HPLC method to determine atenolol concentration is a good method. The two formulations were bioequivalent, the relative bioavailability was 94.22%.