Study on the bioavailability of Atenolol tablet

Objective:To establish a method to determine atenolol concentration in plasma by SPE-HPLC,and to study relative bioavailability of atenolol tablet.Methods:The atenolol concentrations in plasma through solid-phase extraction were determined by HPLC method following a single oral dose were given to 12 volunteers.Results:RSD of intra-day and inter-day were less than 10% and recovery rates of plasma coincided for pharmacokinetic study.The pharmacokinetic parameters of experimental tablets and comparative tablets AUC 0～36 ?C max ?T max ?t 1/2 was 5150 35±1172 52ng·h/ml and 5466 50±1277 08ng·h/ml,717 04±189 69ng/ml and 678 63±193 36ng/ml,2 42±0 45h and 2 63±0 54h,7 84±1 25h and 7 73±1 59h.There were no significant different between the two in formulations in the AUC T max ?C max .Conclusion:The SPE-HPLC method to determine atenolol concentration is a good method.The two formulations were bioequivalent,the relative bioavailbility was 94 22%.