Relative Expression of A-FABP and H-FABP Gens in Chicken Measured by Real-Time PCR

The relative expression in heart and adipocyte fatty acid-binding protein(H-FABP and A-FABP) genes was detected using real time quantitative RT-PCR in Rugao and Anka chicken.The primers were designed according to the sequences of H-FABP,A-FABP and GAPDH gene.GAPDH gene was the internal reference gene.The cDNA fragment was amplified from leg muscle,breast muscle,cardiac muscle,liver and abdominal fat mRNA;H-FABP,A-FABP and GAPDH gene expression was detected using SYBR Green I real-time fluorescent quantity PCR.Melting curve analysis showed a single peak of H-FABP,A-FABP gene and GAPDH gene.PCR efficiency of H-FABP,A-FABP and GAPDH was 99.7%,99.8% and 100.0% respectively.The relative H-FABP mRNA level(ratio of Anka to Rugao) in cardiac muscle was 0.0860,0.0680 and 0.0580,significantly higher than that of the other tissues(P 0.01).H-FABP mRNA expression level was significantly negative correlation with IMF contents.The relative A-FABP mRNA level(ratio of Anka toRugao) in abdomen fat and liver was 15.9640 and 10.9640,significantly higher than that of the other tissues(P 0.01).The relative A-FABP mRNA level in abdomen fat was higher than that of Liver.There were no significant differences between leg muscle,breast muscle and cardiac muscle of relative A-FABP mRNA levels.