Study on the relative H-FABP and A-FABP mRNA gene expression in Qingyuan partridge.

【Objective】 Heart fatty acid-binding protein(H-FABP) and adipocyte fatty acid-binding protein and(A-FABP)gene expression of different tissues were analysed in Qingyuan patridge chicken at different ages.The study will establish a basis for clarifying H-FABP and A-FABP gene expression mechanism in IMF and abdomen fat deposition.【Method】 The primers were designed according to the sequences of H-FABP,A-FABP and GAPDH gene.The c-DNA fragment amplified from leg muscle,breast muscle,cardiac muscle,liver and abdominal fat was used as a standard template;H-FABP and A-FABP gene expression was detected using SYBR Green I real-time fluorescent quantification PCR.The relative expression in H-FABP,A-FABP gene was detected using real time quantitative RT-PCR in Qingyuan patridge chicken.【Result】 Melting curve analysis showed a single peak of the genes.IMF content in breast muscle and leg muscle of Qingyuan partridge on 120 d was higher than that of 56 d.However,the relative H-FABP mRNA level in these tissues was significantly lower than that of Qingyuan partridge on 56 d.The relative H-FABP mRNA level in Cardiac muscle was significantly higher than that of breast muscle and leg muscle.The relative A-FABP mRNA levels of abdomen fat and liver were significantly higher than that of the other tissues(P0.05).The relative A-FABP mRNA level in abdomen fat was higher than that in liver.H-FABP mRNA expression level was significantly postive to IMF contents.A-FABP mRNA expression level was significantly negative to abdomen fat and liver.【Conclusion】 H-FABP gene mRNA was expressed in IMF of different muscle tissues.Moreover,the relative H-FABP mRNA level was much higher in Cardiac muscle.H-FABP mRNA expression level was significantly positive to IMF.Furthermore,it had negative effect on IMF content.A-FABP gene mRNA was expressed in abdomen fat and liver.Moreover,the relative A-FABP mRNA levels in abdominal fat was higher.A-FABP mRNA expression level was significantly negative to abdominal fat.A-FABP mRNA expression level had a significantly positive effect on abdominal fat rate.