Protective effects of Xiaokening granules on endothelial cells induced by high glucose

Objective To investigate the protective effect of Xiaokening granules on umbilical vein endothelial cells(ECV-304) induced by high glucose.Methods ECV-304 cell were cultured in vitro at different concentrations of Xiaokening and high glucose.①Observations of the ECV-304 cells stained with hematoxylin and eosin(HE) was performed for morphological changes.②The proliferation activity of ECV-304 cells cultured in vitro at different concentrations was determined by using MTT.Results ①It was found that ECV-304 cells in high glucose control group were inhibited in growth and became small and round,with varied vacuolar degenerations.In each of Xiaokening groups,the ECV-304 cells exhibited recovery to certain extent.②In Xiaokening groups,in contrast to high glucose group,inhibitory effect on the proliferation of ECV were reduced,especially in 80 g/L and 100 g/L groups(48 hours groups) and the differences were considered statistically significant(P0.05).Conclusion High glucose causes injury to ECV-304 cells and remarkably suppresses their proliferation,while Xiaokening can effectively reduce the cellular injury and proliferation inhibition resulting from high glucose to ECV-304,hence rendering protective effects on endothelial cells.