Proliferation and Apoptosis of Vascular Endothelial Cells under High Glucose Concentration

Objective:To investigate the effect on proliferation and apoptosis of vascular endothelial cells exposed to high glucose. Methods:The cultured human umbilical vein endothelial cells (HUVEC) were treated with high glucose at concentrations of 10,20,30,40,50mmol/L for 2,4,6,8,10,12 and 14 days,cpm value was studied by using tritium-labelled thymidine deoxyribose ( 3H-TdR) incorporation assay.Flow cytometry was used to detect proliferation index,apoptosis index and cell cycle.Results:Treated with high glucose,the cpm value was significantly reduced (P0001),The proliferation index induced by high glucose was much lower than that of the control groups(P005)which were dependent on the dose and the time course.The apoptosis index of HUVEC in most groups was higher in dose dependent manner compared with that of the control groups(P005).The percent of the cultured cells in G1 phase in all high glucose groups were increased and that of the S phase was greatly decreased(P005)in a dose-time dependent manner.Conclusion:Exposed to high glucose,the apoptosis of HUVEC was accelerated and the suppressive effect of high glucose on the proliferation of HUVEC was observed.From the fact that the cells in G1 phase were increased but in S phase were decreased,the endothelial cells were possibly arrested in G1/S checkpoint was suggested.