Acidolysis of Lard by Lipase to Produce Reduced-calorie Functional Lipids

Rich and inexpensive fats and oils from animals and plants,which are readily available in China,have not found effective utilization.In order to make better use of these resources,lipase-catalyzed acidolysis of lard with caprylic acid to produce functional lipids was investigated.It was found that among the five lipases that were tested in the initial screening,immobilized lipase TL IM from T.languginosa was the best catalyst for the incorporation of caprylic acid into lard.The effects of enzyme loading,organic solvent,(substrate) ratio, reaction time,and temperature on the acidolysis reaction were further studied by HPLC analysis of the products.The results showed that the highest caprylic acid incorporation ratio,(34.9±1.7)%,was(obtained) in hexane at 15% of enzyme loading,1∶[KG-*3/5]2 molar ratio of lard to caprylic acid and 55 ℃ for 24 h,and a(caprylic) acid incorpration ratio of(32.4±2.6)% at 20% of enzyme loading,1∶[KG-*3/5]2 molar ratio of lard to caprylic acid and 50 ℃ for 24 h in no solvent system was obtained.