Lipase Modification of Lard to Produce Functional Lipid

China has rich and inexpensive fat and oils from animal and plants, but these resources could not be effectively utilizated. In order to make use best of these resources, the lipase-catalyzed acidolysis of lard with caprylic acid to produce functional lipid was investigated. Of the five lipases that were tested in the initial screening, immobilized lipase TL IM from T. languginosa resulted in the highest incorporation of caprylic acid into lard. This enzyme was further studied for the effects of enzyme load, organic solvent, substrate ratio, reaction time and temperature respectively. HPLC the products from the acidolysis reactions were analyzed. The results showed that the optimal reaction conditions for the highest incorporation of caprylic acid were as follows: hexane was the reaction medium; enzyme load was 15 %(Wt % of substrates); mole ratio of substrates was 1: 2 (lard: caprylic acid); reaction time and temperature were 24h and 55～60℃ respectively.