Effect of Benzo(a) Pyrene on viability and lipid peroxidation of neuron

Objective To investigate the effects of B(a)P on neuronal viability,SOD activity and MDA content in neuron.Methods Primary neurons were dissociated from cerebral cortex of 1-3 d-old SD rats and cultured with DMEM incubator at 37 ℃,and cell density was adjusted at 1×105cells/ml.After 5 d culture,the neurons were divided into two groups:group 1 [treated by B(a)P with S9] and group 2 [only by B(a)P].According to B(a)P concentrations in culture medium,the neurons in each group-were divided into four subgroups:0,10,20,and 40 μmol/L subgroups.And then,the neurons in all groups were cultured for 40 h.The neuronal viability was determined by Cell Counting Kit 8,and SOD activity and MDA content were determined by xanthine oxidase(XOD)method and thiobarbituric acid(TBA)method.Results In groups 1 and 2,neuronal viability,and SOD activity decreased,and MDA content increased in a dose-dependent fashion.Compared with 0 μmol/L subgroup,there was significant difference in 20 μmol/L,40 μmol/L subgroups in group 2.While in group 1,there was significant difference between three dose subgroups and 0 μmol/L subgroup.Conclusion B(a)P could have cytotoxicity on neurons,which may be caused by lipid peroxidation.