Protective effect of RAPA on rat liver ischemia-reperfusion injury

Objective To observe the effect of rapamycin(RAPA)on liver function in rat liver ischemiareperfusion injury.Methods Rat liver ischemia-reperfusion model was established.Sham group only underwent abdomen opening and dissection of the hepatic portal after anesthesia while operation group had blood supply blocked.RAPA group had intravenousinjection of 5 mg/kg slowly 5 min before liver ischemia and reperfusion.Rats in sham group and operation group were given normal saline of the same volume.At 2,6 and24 h after reperfusion, each group had peripheral serum tested for liver function and liver specimen pathology detected.Results ALT, AST,MDA,TNF-αand IL-1 at different times were significantly higher in operation group and RAPA group than in sham group(P0.05).ALT,AST,MDA,TNF-αand IL-1 at different times were lower in RAPA group than in operation group(P0.05).In operation group,ALT,AST,MDA,TNF-αand IL-1 at 24 h were higher than those at 2 h and6 h(P0.05).ALT,AST,MDA,TNF-αand IL-1 at 6h were higher than those at 2 h(P0.05). In RAPA group,ALT,AST,TNF-αand IL-1 at 6 h and 24 h were higher than those at 2 h(P0.05).SOD activity was lower in operation group and RAPA group than in sham group.SOD at 6 h and24 h was(51.42±5.19) IU/L and(49.11±5.07)IU/L in operation group and(59.17±5.62)IU/L and(58.34±5.20)IU/L in RAPA group,respectively,which were higher than those at 2 h(P0.05).Conclusion RAPA can effectively improve hepatic ischemia-reperfusion injury and thus has a significantly protective effect on ischemia-reperfusion injury in the liver.