Effect of Dexmedetomidine Preconditioning on TNF-α and ICAM-1 Expression in Rats during Liver Ischemia-reperfusion Injury

Objective:To observe the effect of dexmedetomidine preconditioning on hepatic ischemia-reperfusion injury in rats and investigate the role of TNF-α and ICAM-1 in hepatic ischemia-reperfusion injury. Method:Thirty adult male SD rats of weighing 150-220 g were randomly divided into 3 groups of sham-operated group(S group),ischemia-reperfusion group(IR group)and dexmedetomidine group(Dex group),each group of 10 cases. S group and IR group were injected 0.9% saline 1 mL/(kg·h)for 30 min. Dex group were injected dexmedetomidine 5μg/(kg·h)for 30 min. IR group and Dex group animals were administrated hepatic ischemic 1 hours after 2 hours. Then reperfusion was done 4 hours after hepatic ischemia. The animals were killed after reperfusion. Then the specimens of liver tissues and blood were obtained. Serum ALT,AST and the levels of TNF-α and ICAM-1 in the hepatic tissues were detected. The liver tissues were observed with microscopic examination. Result:Compared with S group,ALT,AST and the expression of TNF-α and ICAM-1 were significantly higher in Dex group and IR group. In Dex group,ALT and AST were significantly lower than IR group. The expression of TNF-α and ICAM-1 in Dex group were also significantly lower. Microscopy examination showed the degree of injury in Dex group was smaller than that in IR group. Conclusion:The expression of TNF-α and ICAM-1 could be inhibited by dexmedetomidine preconditioning before hepatic ischemia-reperfusion. The expression of ICAM-1 may be regulated by TNF-α in ischemia-reperfusion injury.