Determination of Adapic Acid in Food by Gas Chromatography

A method was established for the determination of adipic acid residue in six kinds of foods using gas chromatography with flame ionization detection(FID).The adapic acid was extracted with ethyl acetate through the acid-assisted extraction.The extract was evaporated to dryness and derivatized with N,O-bis(trimehylsilyl) trifluoroacetamide(BSTFA).The influences of temperature,time,derivatization reagent and its amount on the silylation derivatization efficiency were investigated and optimized.Then the contents were examined with pimelic acid as an internal standard.The limit of detection was 5 mg/kg,and the calibration curve was good linear in the concentration of 5~600 μg/mL with the correlation coefficient more than 0.999.The method was applied to the analysis of adapic acid in the six kinds of food samples and the average recoveries were in the range of 80%~106% with the relative standard derivations of less than 7%.This method could be used to determine the adapic acid in import and export foods.