Analysis of peripheral blood cell subsets in patients with lung cancer and its relationship with clinic

Objective To assay the distribution of T cell subsets,NK cells in peripheral blood of lung cancer patients and investigate the relationshiop between them and the clinical stage and chemotheropy of lung cancer patients.Methods There were 89 lung cancer in-patients and 30 healthy examined persons from June 2009 to July 2010,and the proportions of CD+3,CD+4,CD+4/CD+8 T cells and CD+16 CD+56,CD+4 CD+25,CD+8 CD+28 cells in peripheral blood detected by flow cytometry(FCM) and the level of CEA of them were analyzed retrospectively.Results There were no significant difference of the distribution of cell subsets in periheral blood between lung cancer patients who were in phase of Ⅰ-Ⅱ stage and had different pathological differentiation of cancer and normal level of CEA and healthy controls.For lung cancer patients who were in the phase of Ⅲ~Ⅳ stage and had high level of CEA,the CD+4 and CD+8 CD+28 value and the ratio of CD+4/CD+8 decreased(P0.05),CD+8 and CD+4 CD+25 value increased(P0.05) compared to healthy controls and the activity of NK cells showed no significant difference.Two weeks after patients suffered from chemotheropy,the CD+4 and CD+8 CD+28 value and the ratio of CD+4/CD+8 decreased(P0.01),CD+8 and CD+4CD+25value increased(P0.01) and the ratio of NK cells increased(P0.01).Conclusions The immune function of Lung cancer patiens has some degree of suppression especially when they are in the phase of Ⅲ~Ⅳ stage and have high level of CEA.There is no improvements of the distribution of cell subsets after chemotherophy,and even gets worse.