Study of dendritic cells acquiring antigen from apoptotic hepatochlangioma cells caused by antisense bcl-2 expression

Objective To study relation between bcl 2 and tumor cell, a retroviral vector encoding antisense bcl 2 gene was transfected into tumor cell to cause apoptosis efficiently presented of antigen by human dentritic cells (DCs) from peripheral blood.Methods Using granulocyte/macrophage colony stimulating factor (GM CSF) and interleukin 4 (IL 4), DCs were obtained from peripheral blood monocyte cells that maintained the antigen capturing and processing capacity characteristic in vitro. GM CSF 50 μg/L and IL 4 1?000 U/ml were added once two days for 4 times. On the 3rd day of culture, the immature DCs and apoptotic hepatochlangioma cells caused by antisense bcl 2 expression were cultured for other 4 days.Results These cells had typical dendritic morphology, expressed high levels of CD1a, B7 and acquired antigen from apoptotic cells caused by antisense bcl 2 expression and obviously induced an T cell stimulatory capacity in MLR.Conclusion DCs were obtained from blood monocytes with GM CSF and IL 4, and DCs can efficiently present antigen derived from apoptotic cells caused by antisense bcl 2 expression and induce T cells increasing obviously. It probably become an effective approach of DCs transduced with antigen.