Study on Dendritic Cells Acquiring Antigen from Apoptotic Hepatochlangioma Cells Caused by γ-Ray

Objective：The aim of this study was to establish efficient presentation of antigen from apoptotic cells caused by γ-ray with human dendritic cells (DCs) from peripheral blood. Methods：The authors have obtained DCs from peripheral blood monocyte cells that maintain the antigen capturing and processing capacity characteristic in vitro, with granulocyte/macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4). GM-CSF 50 ng/ml,IL-4 1000 μg/ml once two days, total four. From the 3 rd day of culture, immature DCs and apoptotic hepatochlangioma cells caused by γ-ray were in coculture for 4 days. Results：These cells have typical dendritic morphology, express high levels of CD1a, B7, and acquire antigen from apoptotic cells caused by γ-ray and obviously induce an T cell stimulatory capacity in MLR. Conclusions：The authors have achieved DCs from blood mononuclear using GM-CSF and IL-4 and DCs can efficiently present antigen drived from apoptotic cells caused by γ-ray and induce T cells increasing obviously. It probably become effective approach of DCs transduced with antigen.