Study on Genetic Alterations in Chromosomes 8p and 16q in Primary Hepatocellular Carcinoma

Objective:To investigate the genetical alterations on chromosomes 8p and 16q in primary hepatocellular carcinoma(HCC),investigate the relationship between the gentical alterations and clinicopathologic features and try to screened some HCC-related tumor suppressor genes.Methods:Loss of heterozygosity(LOH) and microsatellite instability(MSI) on chromosome 8p and 16q in samples from thirty-five patients with HCC were examined by PCR-denaturing PAGE-silver staining.Results:The overall LOH frequency was 68.89%(31/45) at least one locus of 8 loci on the chromosomes.The frequency of Loci were 53.33%(31/45),39.02%(16/41)and34.88%(15/43)for D16S511,D8S261 and D8S499,respectively.The frequency of MSI was 11.11%(5/45) and the MSI was distributed on the three microsatellite markers(D16S511、D8S261 and D8S499).Conclusion:There may be a new putative tumor suppressor gene related to the occurrence and development on specific chromosome region 16q23,8p22-21.3 or 8p12 with high-frequent LOH.The genetic alterations on some specific loci were associated with prognosis factors as the positive HBsAg,differentiated degrees of HCC.