Ioss of heterozygosity on chromosomes 8 and 16 in primary hepatocellular carcinoma

Objective:To study the loss of heterozygosity on chromosomes 8p and 16q in primary hepatocellular carcinoma(HCC),evaluate the relationship between the loss and clinicopathologic features and try to screened some HCC-related tumor suppressor genes in order to provide possible molecular markers for early diagnose and prognosis warning of HCC screening.Methods: The frequency of loss of heterozygosity(LOH) at some specific microsatellite loci on chromosome 8p and 16q in tissue samples from thirty-five patients with HCC were examined by using PCR-denaturing PAGE-silver staining.Results: The overall LOH frequency was(68.89%,31/45) at least one locus of 8 loci on the chromosomes.The three most more frequent loci were D16S511(53.33%,31/45),D8S261(39.02%,16/41)and D8S499(34.88%,15/43).Conclusion: There may be a new putative tumor suppressor gene related to the occurrence and development on specific chromosome region 16q23,8p22-21.3 or 8p12 with high-frequent LOH.The genetic alterations on some specific loci were associated with such prognosis factors as the positive HBsAg,differentiated degrees of HCC.