2010•Zhongguo yufang shouyi xuebaoRequires access
The protective efficacy of the inactivated H5N1 subtype avian influenza virus vaccine Re-4 in chickens, ducks and geese.
Xianying Zeng, Gongxun Zhong, Yanbing Li, Jianzhong Shi, Jiang Yongping, Yanni Wang, Xiaopeng Wan, Mao ShengGang, Tian GuoBin, Hualan Chen
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Abstract
The protective efficacy of the inactivated avian influenza virus(AIV) H5N1 subtype vaccine(Re-4) derived from AIV H5N1 A/Chicken/Shanxi/2/2006(CK/SX/2/06) were evaluated in chickens,ducks and geese.Chickens,ducks and goose were immunized with Re-4 vaccine and the hemagglutinin-inhibition(HI) antibody was detected on a weekly basis.The SPF chickens were intranasally challenged with 105 EID50 of CK/SX/2/06 at 2,3 and 50 weeks post vaccination(p.v).The results showed that Re-4 vaccine induced strong immune response in chickens,ducks and geese.SPF chickens with a HI antibody titre above 4 log2 were completely protected from challenge.Based on these results,we propose the following vaccination program:layers could be immunized with the initial dose of 0.3 mL at the age of 10-day-old and the second dose of 0.5 mL at the age of 60-day-old,andthe third dose of 1.0 mL at the age of 110-day-old;ducks and geese could be immunized with the initial dose of 0.5 mL at the age of 2-week-old and the second dose of 1.0 mL at the age of 5-week-old,and the third dose of 1.0 mL at the age of 4-month-old.