Screening single nucleotide polymorphisms in the predisposing gene-ERCC2 of gliomas by denaturing high-performance liquid chromatography

Objective To screen single nucleotide polymorphisims(SNPs)of ERCC2 gene at 19q13.3 by way of denaturing high-performance liquid chromatography(DHPLC).Methods Twenty-second exon and the adjacent part of the intron in the ERCC2 gene at 19q13.3 were isolated from the glioma and the blood samples of 179 patients with gliomas and 44 healthy control groups were amplified by PCR,and the products were analyzed by D1HPLC according to the primer sequence of the literature report.Some meaningful fragments were sequenced and compared with the sequences available from National Center for Biotechnology Information(NCBI) database.Results In this study,1 new SNP loci was preliminary identified and 1 known SNP loci and genotype was confirmed,moreover,1 known SNP loci and genotype,which occurred in white tumor patients,was eliminated.Conclusion SNPs loci has the race discrepancy.DHPLC is an effective,economical,and simple method with reliability for SNPs screening.