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Single Nucleotide Polymorphisms Detection by Denaturing High Performance Liquid Chromatography in the Chicken HSP70 Gene

Luo Qing-bin, Zeng Fantong

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Abstract

In this research, 591 individuals from 20 different Chinese native chicken breeds were studied. By using DHPLC technology Unknown SNPs were detected in the chicken HSP70 gene sequence and for every mutant chromatographic types, 2 individuals were selected to sequence. Homologous analysis was carried out based on the sequence information of samples that amplified from same primer to identity new unknown SNPs. In the PCR products amplified by primer A5, A6, A7 and A4, 10 SNPs sites (A258G, C276G, C507T, C1040A, G1044A, C1431A, T1476C, G1500A, A1529G and C1722T) are detected.

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What this paper is about

In this research, 591 individuals from 20 different Chinese native chicken breeds were studied. By using DHPLC technology Unknown SNPs were detected in the chicken HSP70 gene sequence and for every mutant chromatographic types, 2 individuals were selected to sequence. Homologous analysis was carried out based on the sequence information of samples that amplified from same primer to identity new unknown SNPs. In the PCR products amplified by primer A5, A6, A7 and A4, 10 SNPs sites (A258G, C276G, C507T, C1040A, G1044A, C1431A, T1476C, G1500A, A1529G and C1722T) are detected.

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Available abstract

In this research, 591 individuals from 20 different Chinese native chicken breeds were studied. By using DHPLC technology Unknown SNPs were detected in the chicken HSP70 gene sequence and for every mutant chromatographic types, 2 individuals were selected to sequence. Homologous analysis was carried out based on the sequence information of samples that amplified from same primer to identity new unknown SNPs. In the PCR products amplified by primer A5, A6, A7 and A4, 10 SNPs sites (A258G, C276G, C507T, C1040A, G1044A, C1431A, T1476C, G1500A, A1529G and C1722T) are detected.

Key concepts: Single-nucleotide polymorphism, Biology, Primer (cosmetics), Denaturing high performance liquid chromatography, Gene, Genetics, Molecular biology, Gene sequence

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