Effects of Matrine on Cisplatinum Sensitivity of Epithelial Ovarian Cancer and Analysis of Its Potential Mechanism

Objective To explore the effect of matrine on the cisplatinum sensitivity of epithelial ovarian cancer cell and the mechanism from the apoptosis resistance.Method The OVCAR3 cell line were cultured and divided into two groups,the experiment group was treated by matrine with three different concentrations(10,40,160μmol/L).MTT served to test the platinum sensitivity of both groups,the ELISA was used to determine the activity of NF-κB,the Western-blot was used to determine the expression of Livin and Survivin.Results The cisplatinum sensitivity of both groups was statistically different,the IC50 of experiment groups(10,40,160μmol/L matrine treated)were(29.75±5.27),(25.61±4.27),(17.29±3.35)μmol/L respectively,which is statistically lower than that of control group(31.65±2.23μmol/L).The NF-κB expression of experiment groups(10,40,160μmol/L matrine treated)were(0.507±0.027),(0.375±0.019),(0.311±0.017),which was statiscally lowere than that of control group(0.705±0.032).Matrine treatment can decreased the expression of Livin and Survivin,the in?uence is correlated with the concentration of matrine.Conclusion The treatment of matrine treatment can increase the cisplatinmu sensitivity of ovary epithelial cell.The variance of signal pathway of NF-κB,Livin,Survivin is one of the mechanism.