Simultaneous determination of organic acid and glucose in succinic acid fermentation broth of actinobacillus succinogenes by reversed-phase high performance liquid chromatography (RP-HPLC)

A RP-HPLC method for simultaneous determination of organic acid and glucose in succinic acid fermentation broth of Actinobacillus succinogenes was developed. Eclipse XDB-C_8 column(4.6 mm i.d.×150 mm, 5 μm) was used and the mobile phase was 0.005 (mol/L) sulfic acid solution (pH 2.5) with flow rate of 1.0 mL/min. Organic acid and glucose in samples were determined by refractive index detector (RID) within 9 min. The regression equation, the linear range, the correlation coefficient and the detection limit were determined. The relative standard deviation (RSD, n =5) was in the range of 0.41%～0.89%, and the average recovery was in the range of 99.6%～100.8%.