Bioavailability study of ciprofloxacin hydrochloride sustained-release capsules in dogs

Objective To study on the pharmacokinetics and bioavailability of ciprofloxacin hydrochloride sustained-release capsules on comparison with that of the conventional tablets.Methods Acetonitrile was added to the plasma to sediment the protein and then chloroform was added to remove the impurity and the part of acetonitrile. The concentration of ciprofloxacin hydrochloride in dogs′ plasma was determined by RP-HPLC method.The plasma concentration-time curves were plotted.The main pharmacokinetic parameters of the two preparations and the relative bioavailability of the sustained-release capsules were obtained.Results The c _ max of the two preparations were (9.20±2.02)mol·L -1 and (22.51±7.24)mol·L -1 respectively; t _ max if that was(6.00±0.0)h and (2.50±0.55)h and t _ MR were (12.23±2.92)h,(5.66±1.7)h respectively.There was no significant difference between the AUC value of the two formulations.The oral relative bioavailability of the sustained-release capsules to the conventional tablets was (97.10±9.61)%.Conclusion By the statistical analysis,the ciprofloxacin hydrochloride sustained-release capsule possessed good sustained-release characeteristics and the test and reference formulations were of bioequivalence.Significant correlation between in vitro dissolution and in vivo absorption of ciprofloxacin hydrochloride sustained-release capsules was found.