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Determination of Total Alkaloid in Zanthoxylum nitidum(Roxb.)DC. by Bromophenol Blue Colorimetry

Xing Peng

Abstract

The content of total alkaloid in Zanthoxylum nitidum(Roxb.) was determined by bromophenol blue colorimetry, and the UV-visible spectrophometry was adopted to determine the total alkaloid with the wavelength at 393 nm. The total alkaloid is in linear relationship within the concentration range of 2.56～ 10.26μg/ml(r=0.9997), the average recovery rate is 101.00% (RSD=2.46%, n=6). Bromophenol blue colorimetry is simple, quick and accurate in the determination of total alkaloid in ZanthoxyLum nitidum (Roxb.)DC.

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Key concepts: Bromophenol blue, Colorimetry, Alkaloid, Chemistry, Chromatography, Zanthoxylum, Traditional medicine, Stereochemistry