2005•Chinese Journal of Geriatric Cardiovascular and Cerebrovascular DiseaseRequires access
Protective effect of naloxone on mitochondrial function of hippocampal cells injured by ischemia-reperfusion
Hong Shen
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Abstract
Objective To study the effect of naloxone on the hippocampal cells of rabbit injured by ischemia and reperfusion.Methods Twenty white rabbits were randomly divided into four groups:control group, ischemia group, reperfusion group and naloxone group. At the end of intervention, the concentration of free calcium ion and mitochondrial membrane potential of hippocampal cells were assayed by flow cytometry.Results The concentrations of free calcium ion of both ischemia group and reperfusion group were significantly higher than those of control and naloxone groups(P0.01 respectively).Their mitochondrial membrane potentials were lower than those of the latters(P0.01 respectively).The concentration of free calcium ion of reperfusion group was higher than that of ischemia group and its mitochondrial membrane potential was lower than that of ischemia group(P0.01).There were no remarkable differences in the concentration of free calcium ion and the mitochondrial membrane potential between the naloxone group and control group.Conclusions Ischemia and reperfusion may lead to rise in concentration of free calcium ion, loss of mitochondrial membrane potential of hippocampal cells. Naloxone may significantly inhibit aforementioned changes. It has protective effect on the mitochondrial function of the hippocampal cells.