Nalxone decreases the ratio of apoptosis of the rabbit’s hippocampus cells induced by ischemia and reperfusion

Objective:To study the protective effect of Nalonxe on the hippocampus cells of rabbits induced by ischemia and reperfusion. Methods:20 New Zealand white rabbits were randomly divided in four groups: control group, ischemia (bilateral common carotid artery and vertebral artery were occluded for 30mins) group, ischemia-reperfusion(above mentioned occluded artery were opened for 6hours) group, reperfusion associated with Naloxone therapy(0.8mg/kg iv and then 0.3mg/kg vd.) group. At the end of intervention, the ratio of apoptosis of hippocampus cells were assayed by flow cytometry. Results:1.The ratio of apoptosis of ischemia group and ischemia-reperfusion group were all significant higher than control group(P0.01 respectively); and the ratio of ischemia-reperfusion group was significant higher than ischemia and naloxone group(P0.01 respectively). (2. There) was no remarkable difference between that of naloxone therapy group and control group.Conclusion:Ischemia and ischemia-reperfusion may lead to apoptosis of hippocampus cells. Naloxone significantly reduces apoptosis of hippocampus cells and provide protective effect on hypoxia and reperfusion injure.