Evaluation on antioxidant activity of Ceropegia dolichpohylla extracts in vitro

Objective:To evaluate on antioxidant activities of extracts from Ceropegia dolichpohylla in vitro. Methods:Salicylic acid method,DPPH·(2,2-diphenyl-1-picrylhydrazyl)method and deoxidization Fe3+ were used to evaluate the antioxidant activities in vitro of samples,which extracted using ethanol,petroleum ether,chloroform,ethyl acetate,n-butanol and water from Ceropegia dolichpohylla,respectively,and VC was used as comparison sample. Results:The extracts had good scavenging activities for hydroxyl radical(·OH),especially crude polysaccharide showed the highest activity and its half elimination ratio(IC50)was 0. 61mg/mL,but the activity was weaker than that of VC. The extracts had strong scavenging activities on DPPH radical,the effect of ethyl acetate extracts was the strongest and its IC50 value was 0. 13mg/mL. Meanwhile,the above extracts had stronger deoxidizing Fe3+ activity,the activity of the petroleum ether extracts was the strongest. Conclusion:Ceropegia dolichpohylla has stronger antioxidative activity and will be widely applicated on antiaging.