Expression of CD2AP mRNA in rats with adriamycin nephropathy

Objective To investigate the expression of CD2-associated protein (CD2AP) mRNA in rats with adriamycin nephropathy, and to explore the possible effect on the development of proteinuria.Methods Thirty SD rats, weighted from 140 to 160g,were randomly divided into the nephropathy model groups and normal control group. The nephropathy model was established by a single intravenous injection of adriamycin 6mg/kg.While the normal control group received the same volume of 0.9% sodium chloride. At the end of the 2 nd, 4 th, 6 th or 8 th week after injection, the rats were sacrificed and the kidneys were acquired. The renal histology was observed under light microscope and electronic microscope. The semiquantitative RT-PCR was used for detecting the levels of CD2AP mRNA.Results (1)CD2AP mRNA levels in the 4th,6th and 8th week groups were 1.7, 2.5 and 1.9 times those in normal control group respectively.(2)The expression of CD2AP mRNA was positively correlated with the amount of proteinuria (r=0.56,P0.01).Conclusion The increased expression of CD2AP mRNA is to some extent related to the development of proteinuria in adriamycin nephropathy rats.