EXPRESSION OF WT1 IN RATS WITH ADRIAMYCIN NEPHROPATHY

Objective To investigate the expression of WT1(Wilms′tumor 1) in rats with adriamycin nephropathy,and to explore the possible effect on the development of proteinuria.Methods 30 SD rats,from 140 to 160g,were randomly divided into the nephropathy model group and normal control group.The nephropathy model was established by a single intravenous injection of adriamycin 6 mg/kg while the normal control group received the same volume of 0.9% sodium chloride solution.At the end of 2nd,4th,6th and 8th week after injection,the rats were sacrificed and their kidneys were acquired.The renal histology was observed with light microscope and electronic microscope.The semiquantitative Western Blot was used to detect the levels of WT1.Results① The protein levels of WT1 in the nephropathy group were 1.5,2.7,3.1 and 4.3 times respectively as high as those in the normal control group.② The expression of WT1 was positively correlated with the amount of proteinuria(r=0.95,P0.001).Conclusions The increased expression of WT1 was closely associated with the development of proteinuria in adriamycin nephropathy rats.