Effect of Xiao Wu Gui granule drug-containing serum on the proliferation of CIA rats knee fibroblast-like synoviocytes in vitro

Objective To evaluate the effect Xiao Wu Gui granule drug-containing serum on the proliferation of CIA rats fibroblast-like synoviocytes (CIA-FLS) in vitro. Methods CIA rat model that was induced with chicken collagen type Ⅱ,uses the arthritis index score and pathological inflammation scoring system for evaluation of the degree. CIA-FLS were isolated and cultured from the synovium of CIA rats by tissue block culture method ,and then respectively treated with methotrexate (MTX),different concentrations of drug-containing serum or nothing (control group) for 24,48,and 72h, the changes in the cell proliferation were detected by MTT assay ,and the changes in the cell supernatant of TNF-α and MMP-1 cytokine concentrations were detected by ELISA assay. Results The high/medium dose drug-containing serum and MTX suppressed the CIA-FLS proliferation and reduced the TNF-α and MMP-1 cytokine concentration at 24 ,48,and 72h after the exposure (P0.05). The low dose drug-containing serum suppressed the CIA-FLS proliferation and reduced the TNF-α cytokine concentration at 48 and 72h after the exposure (P 0.05).The low dose drug-containing serum reduced the MMP-1 cytokine concentration at 24 ,48,and 72h after the exposure (P0.05). Conclusion Xiao Wu Gui drug-containing serum can inhibit FLS proliferation and secreted TNF-α and MMP-1 cytokine.