Sudy on Cellulase Enzymolysis Assisted Ethanol Extraction of Total Flavonoids from Radix Astragali

Cellulase enzymolysis assisted ethanol extraction of astragalus total flavonoids from Astragalus membranaceus was optimized in the paper. To observe and study this,the effects of factors including enzymatic content,enzymolysis time,solid-to-liquid ratio,ethanol concentration,extraction time,extraction temperature on the extraction rates of astragalus total flavonoids were explored by single-factor and orthogonal test. The results showed that solid-to-liquid ratio was the most critical factor affecting the extraction rate of astragalus total flavonoids,followed by ethanol concentration and extraction temperature,extraction time exhibited less effect on extraction rate of astragalus total flavonoids. The optimal extraction conditions were solid-to-liquid ratio of 30mL / g,ethanol concentration of 90% ,extraction temperature of 75℃ ,extraction time of 2. 5h after cellulase enzymolysis pretreatment in the conditions of enzymatic content of 7. 5μL / g,enzymolysis pH4. 0,enzymolysis temperature 55℃ ,enzymolysis time of 1. 5h,astragalus total flavonoids extraction was up to 0. 57% and improving 46. 15% compared with the traditional ethanol extraction of 0. 39% .