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Rapid Detection of Enterobacter sakazakii by LAMP

Liu Zhiguo

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Abstract

Objective: To develop a loop-mediated isothermal amplification (LAMP) method for rapid detection of Enterobacter sakazakii in food. Methods: LAMP primers were designed on the basis of 16S rRNA gene (16S rDNA) and outer membrane protein A gene (OmpA) in Enterobacter sakazakii. The LAMP products were evaluated by electrophoresis or SYBR-GREEN I. Results: The detection limit of Enterobacter sakazakii by LAMP assay was 32 CFU/mL using 16S rRNA primers or 3 CFU/mL using OmpA primers. The similar detection sensitivity of LAMP products using SYBR-GREEN I and electrophoresis was observed. Conclusion: LAMP method was effective and sensitive for rapidly detecting Enterobacter sakazakii, which will have a broad application prospect in food.

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What this paper is about

Objective: To develop a loop-mediated isothermal amplification (LAMP) method for rapid detection of Enterobacter sakazakii in food. Methods: LAMP primers were designed on the basis of 16S rRNA gene (16S rDNA) and outer membrane protein A gene (OmpA) in Enterobacter sakazakii. The LAMP products were evaluated by electrophoresis or SYBR-GREEN I. Results: The detection limit of Enterobacter sakazakii by LAMP assay was 32 CFU/mL using 16S rRNA primers or 3 CFU/mL using OmpA primers. The similar detection sensitivity of LAMP products using SYBR-GREEN I and electrophoresis was observed. Conclusion: LAMP method was effective and sensitive for rapidly detecting Enterobacter sakazakii, which will have a broad application prospect in food.

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Available abstract

Objective: To develop a loop-mediated isothermal amplification (LAMP) method for rapid detection of Enterobacter sakazakii in food. Methods: LAMP primers were designed on the basis of 16S rRNA gene (16S rDNA) and outer membrane protein A gene (OmpA) in Enterobacter sakazakii. The LAMP products were evaluated by electrophoresis or SYBR-GREEN I. Results: The detection limit of Enterobacter sakazakii by LAMP assay was 32 CFU/mL using 16S rRNA primers or 3 CFU/mL using OmpA primers. The similar detection sensitivity of LAMP products using SYBR-GREEN I and electrophoresis was observed. Conclusion: LAMP method was effective and sensitive for rapidly detecting Enterobacter sakazakii, which will have a broad application prospect in food.

Key concepts: Loop-mediated isothermal amplification, Enterobacter, 16S ribosomal RNA, SYBR Green I, Detection limit, Biology, Microbiology, Bacteria

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