Effect of Atorvastatin on IL-6 Induced Human Umbillcal Vein Endothelial Cell Expressed Endostatin

Objective To study the effect of atorvastatin on IL-6 induced human umbilical vein endothelial cells (HUVECs) expressed endostatin.Methods HUVECs were treated with IL-6 and/or atorvastatin for 24～48 hours.The concentration of endostatin in supernatants was determined by ELISA assay.Endostatin mRNA expression was detected by RT-PCR.Results As compared with control group and IL-6 treatment for 24 hours,the concentrations of endostatin in supernatants elevated significantly after IL-6 treatment for 48 hours (18.45±6.80 vs 6.81±2.33 ng/ml,18.45±6.80 vs 7.07±2.54 ng/ml,both P0.01).As compared with IL-6 treatment for 48 hours,the concentrations of endostatin in cultured cells decreased significantly after At + IL-6 treatment for 48 hours (9.82±4.74 vs 18.45±6.80 ng/ml),and the difference was statistically significant (P0.05).As compared with control group,endostatin mRNA expression in cultured cells increased significantly after IL-6 treatment for 48 hours (0.587±0.220 vs 0.122±0.096,P0.001).As compared with IL-6 treatment for 48 hours,endostatin mRNA expression in cultured cells decreased after At + IL-6 treatment for 48 hours (0.310±0.205 vs 0.587±0.220),and the difference was statistically significant (P0.05).Conclusions IL-6 was potent stimulus of endostatin expression and release on HUVEC.Increased endostatin expression and release induced by IL-6 was inhibited by atorvastatin.