The clinicopathological sigificance of expression of mtp53, Bcl-2, p16 gene and protein in primary lung cancer

Objective: To explore the relationship between abnormal expression of mtp53, p16 and Bcl-2 protein and oncogenesis and development of lung cancer. Methods: Expression of mtp53, p16 and Bcl-2 protein was detected in 114 lung cancer tissues by immunohistochemistry. Loss of p16 INK4 gene exon 2(E2) was analyzed using PCR technique in 62 cancer samples with p16 protein-negative stain and 14 paracancerous lung tissues. Results: Overexpression of mtp53 protein was not related to pathological type and histological grade of lung cancer (P0.05), but correlated with lymph node status (P0.05). Postive expression of p16 was closely related to histological grade of lung cancer and lymph node metastasis (P0.05), but not to pathological type (P0.05). The deletion of p16 E2 was 45.2% in nsclc and closely related to lymph node metastasis and histological grade (P0.05). The postive rate of bcl-2 protein expression in sclc was significantly higher than that in nsclc (P0.05). Besides, there is regulation between p53 and p16. Conclusions: (1) mtp53,p16 and Bcl-2 genes may play important roles in the oncogenesis and development of lung cancer. (2) It exists negative regulation between mtp53 and p16 protein.