Detection and multi-drug resistance analysis of extended-spectrum β-lactamase producing Escherichia coli

Objective To investigate the drug resistance,ESBLs-production,specimens sources and clinical distribution of Escherichia coli. Methods Bacteria identification and ESBLs screening were carried out by using automated microbiology analyzer,while the minimum inhibitory concentration was determined by using agar dilution method. Chi square test was used for data analysis. Results A total of 150 Escherichia coli strains were isolated,and the proportions of sensitive bacteria and multiple-drug resistant bacteria were 29. 3% and 25. 3%,respectively. The 150 Escherichia coli strains showed lowest drug resistance rate to amikacin( 8. 7%),and highest drug resistance rate to trimethoprim / Sulfamethoxazole( 54. 0%). The detection rate of ESBLs was 50%. There were significant differences in resistant rates to amikacin,trimethoprim / Sulfamethoxazole,ceftazidime,levofloxacin and nitrofurantoin between the ESBLs-postive group and ESBLs-negative group( P 0. 05). The source of specimens was mainly urine and Escherichia coli infection was frequently found in urology department( 20. 0%). Conclusion Escherichia coli showed drug resistance at different degrees. There was a close relation between ESBLs and the drug resistance of Escherichia coli.