Effects of Tamoxifen Binding with 5-FU on Growth Inhibition,Proliferation and Apoptosis in Human Colorectal Carcinoma Cell Lines

Objective To evaluate the possible effects of tamoxifen(TAM) lonely or binding with 5 FU chemotherapy on growth inhibition,proliferation and apoptosis in cultured SW480,HT29 human colorectal carcinom cell lines in vitro.Methods The growth inhibition effects of the SW480,HT29 were detected with the Tetrazolium(MTT) assay on the basis of TAM alonly and TAM binding with 5 FU.Through the curves of the chemotherapy durgs number effection relation,the effect of TAM was observed for the increase of the chemosensitization,and its influences on proliferation,apoptosis also observed by detecting PCNA and AI.Results Tamoxifen did not inhibit SW480,HT29 growth,TAM binding with 5 FU at some concentrations inhibited cells growth and increased the chemosensitivity.By detecting both PCNA and AI,TAM binding with 5 FU decreased cells proliferation and increased apoptosis with the concentration of TAM increasing and time prolonging.Conclusions Tamoxifen can increase the chemosensitivity of 5 FU in human colorectal cancer SW480,HT29 cell lines in vitro,inhibit cells proliferation and promote cells apoptosis at higher degree.