Effects of rhTIMP-3 and TAM on growth and apoptosis in MCF-7 breast cancer cell in vitro

Objective: To study the effects of rhTIMP-3 and TAM on breast cancer cell line MCF-7.Methods: Estrogen receptor positive(ER+)MCF-7 human breast carcinoma cell line was treated by rhTIMP-3 or TAM or rhTIMP-3 + TAM in vitro.The cell proliferation was evaluated by MTT assay.Distribution of cell cycle and rate of apoptosis were determined by flow cytometry.Results: Both rhTIMP-3 and TAM can inhibit MCF-7 proliferation and induce the apoptosis.The cells were arrested in S and G2/M phase by rhTIMP-3 and G0/G1 phase by TAM along.The inhibition of cell proliferation and the change of cellcycle kinetics induced by rhTIMP-3,TAM were dependent on the does and time.When rhTIMP-3 combined with TAM,the inhibitory effect on proliferation was enhanced,and apoptosis is synergism.Conclusion: Both rhTIMP-3 and TAM can inhibit MCF-7 cells proliferation,and induce its apoptosis.The inhibitory effect on proliferation can be improved when two drugs are combined.