ScholarLens Back to search
2008•Zhongguo yufang shouyi xuebaoRequires access

Recombinant N protein based ELISA for Detection of Antibody against CDV

Zhenrong Li

Open publisher page 0 citations

Abstract

An ELISA assay was developed for detecting CDV using the recombinant N protein of CDV as coating antigen. The N protein was expressed from plasmid pET-N in E.coli Rosetta 2 (DE3) host cell, verified by SDS PAGE and Western blot assays and purified. The ELISA assay was established under optimized antigen concentrations and antibody dilutions. About 270 serum samples were detected by this method and Synbiotics ELISA kit. The agreement ratio between the two methods was 92.4 %. The assay provided a useful tool for epidemiological investigation of Canine Distemper in Canine.

About this research paper

What this paper is about

An ELISA assay was developed for detecting CDV using the recombinant N protein of CDV as coating antigen. The N protein was expressed from plasmid pET-N in E.coli Rosetta 2 (DE3) host cell, verified by SDS PAGE and Western blot assays and purified. The ELISA assay was established under optimized antigen concentrations and antibody dilutions. About 270 serum samples were detected by this method and Synbiotics ELISA kit. The agreement ratio between the two methods was 92.4 %. The assay provided a useful tool for epidemiological investigation of Canine Distemper in Canine.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

An ELISA assay was developed for detecting CDV using the recombinant N protein of CDV as coating antigen. The N protein was expressed from plasmid pET-N in E.coli Rosetta 2 (DE3) host cell, verified by SDS PAGE and Western blot assays and purified. The ELISA assay was established under optimized antigen concentrations and antibody dilutions. About 270 serum samples were detected by this method and Synbiotics ELISA kit. The agreement ratio between the two methods was 92.4 %. The assay provided a useful tool for epidemiological investigation of Canine Distemper in Canine.

Key concepts: Recombinant DNA, Canine distemper, Biology, Antibody, Serial dilution, Molecular biology, Antigen, Virology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Recombinant N protein based ELISA for Detection of Antibody against CDV — Research Paper | ScholarLens