2007Lishizhen Medicine and Materia Medica ResearchRequires access

Quality Standard of Qinggan Tablet

Rong Chen

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Abstract

Objective To establish the quality standard of Qinggan Tablet.Methods TLC was used to identify Radix Isatidis,Herba Artemisiae Scopariae and Radix Glycyrrhizae;and HPLC was used to determine the content of Glycyrrhiziate acid.Agilent Eclipse XDB-C_(18) column(4.6 mm×250 mm,5 μm)with mobile phase of methanol-0.2 mol·L~(-1) ammonium acetate solution-glacial acetic acid(67∶33∶1) was used for the determination of puerarin.Results The TLC for identification was special and the linear range of puerarin was 0.36~1.80 μg and recovery rate was 98.33%,RSD was 1.24%,respectively.Conclusion This method can be used to control the quality of Qinggan Tablet better.

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Objective To establish the quality standard of Qinggan Tablet.Methods TLC was used to identify Radix Isatidis,Herba Artemisiae Scopariae and Radix Glycyrrhizae;and HPLC was used to determine the content of Glycyrrhiziate acid.Agilent Eclipse XDB-C_(18) column(4.6 mm×250 mm,5 μm)with mobile phase of methanol-0.2 mol·L~(-1) ammonium acetate solution-glacial acetic acid(67∶33∶1) was used for the determination of puerarin.Results The TLC for identification was special and the linear range of puerarin was 0.36~1.80 μg and recovery rate was 98.33%,RSD was 1.24%,respectively.Conclusion This method can be used to control the quality of Qinggan Tablet better.

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Available abstract

Objective To establish the quality standard of Qinggan Tablet.Methods TLC was used to identify Radix Isatidis,Herba Artemisiae Scopariae and Radix Glycyrrhizae;and HPLC was used to determine the content of Glycyrrhiziate acid.Agilent Eclipse XDB-C_(18) column(4.6 mm×250 mm,5 μm)with mobile phase of methanol-0.2 mol·L~(-1) ammonium acetate solution-glacial acetic acid(67∶33∶1) was used for the determination of puerarin.Results The TLC for identification was special and the linear range of puerarin was 0.36~1.80 μg and recovery rate was 98.33%,RSD was 1.24%,respectively.Conclusion This method can be used to control the quality of Qinggan Tablet better.

Key concepts: Chromatography, Puerarin, Quality standard, Radix (gastropod), Acetic acid, Ammonium acetate, Chemistry, High-performance liquid chromatography

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