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2008•China Practical MedicineRequires access

The disk diffusion methods for screening pseudomonas aeruginosa producing metallo-β-lactamase

W. Huang

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Abstract

Objective To investigate the feasibility of the disk diffusion methods for screening Pseudomonas aeruginosa producing metallo-β-lactamase.MethodsMetallo-β-lactamases produced by Pseudomonas aeruginosa was detected by using 2-mercaptopropionic acid and EDTA-Na2 in the M-H agar.Results64 Pseudomonas aeruginosa which resistance for Imipenem acid or Ceftazidime have 9 producing metallo-β-lactamase.The proportion of positive is 14.1%.ConclusionThe method is simple,convenient and sensitive for screening metallo-β-lactamase-producing bacteria.

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What this paper is about

Objective To investigate the feasibility of the disk diffusion methods for screening Pseudomonas aeruginosa producing metallo-β-lactamase.MethodsMetallo-β-lactamases produced by Pseudomonas aeruginosa was detected by using 2-mercaptopropionic acid and EDTA-Na2 in the M-H agar.Results64 Pseudomonas aeruginosa which resistance for Imipenem acid or Ceftazidime have 9 producing metallo-β-lactamase.The proportion of positive is 14.1%.ConclusionThe method is simple,convenient and sensitive for screening metallo-β-lactamase-producing bacteria.

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Available abstract

Objective To investigate the feasibility of the disk diffusion methods for screening Pseudomonas aeruginosa producing metallo-β-lactamase.MethodsMetallo-β-lactamases produced by Pseudomonas aeruginosa was detected by using 2-mercaptopropionic acid and EDTA-Na2 in the M-H agar.Results64 Pseudomonas aeruginosa which resistance for Imipenem acid or Ceftazidime have 9 producing metallo-β-lactamase.The proportion of positive is 14.1%.ConclusionThe method is simple,convenient and sensitive for screening metallo-β-lactamase-producing bacteria.

Key concepts: Pseudomonas aeruginosa, Microbiology, Ceftazidime, Imipenem, Medicine, Pseudomonas, Agar, Agar diffusion test

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