Effect of Trichostatin A on the expression of HDACI in K562 cells

Objective:Histone deacetylase(HDAC1)shows a high expression in many cancer cells and TSA can inhibit it the growth of cancer cells. This paper was designed to investigate the expression of HDAC1 of K562 cell and the effect of TSA on their proliferation and apoptosis. Methods: K562 cells were treated with 100~400nmol/L TSA for 6~48h and the growth inhibition rates of K562 cells were measured by MTT. The expression of HDAC1on K562 cell were checked by mRNA、Western blot at 24h various concertrations(50~500nom/L).Results: TSA in selectively inhibite proliferation of K562 cell line in a dose and time dependent manner. Inhibit rate was 23.15%~76.63%(P0.01). of K562 cells The apoptosis rates were 14.38～61.18%by flow cytometery (FCM), the up regulation of HDAC1 expression was observed within 24h after the treatment in TSA by RT-PCR and Western blot. with the increase of concertration and the expression down regulation dose dependent. Conclusion:The expression of HDAC1 plays an important role in the proliferation and apoptosis of K562 cells, which is the inhibition effect of various concentration of TSA on K562 cells.