Effect of Trichostatin A on expression of HDAC1 in K562 cells

Objective It was manifested that Histone Deacetylase( HDAC1) was expressed highly in many kinds of the tumor cells, and Trichostatin A(TSA) could inhibit their growth. The aim of this experiment was to research the expression of HDAC1 in K562 cells and the influence of TSA on their proliferation. Method K562 cells were treated with TSA in concentrations from 50 to 400 nmol/L for 8 to 48 hours. Then the growth activity was assayed by Dipenyltetrazolium bromide (MTT) colorimetric assay. The apoptosis of cells were observed by flow cytometery(FCM). The mRNA and protein′s expressions of K562 cells, which were treated with HDAC1 in various concentrations from 50 to 400 nmol/L for 24 hours, were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. Further more; the effect of TSA on them was assayed in the same way. Results TSA can inhibit the growth of K562 cells and induce the apoptosis of the cells remarkably, and they were in a time-and-dose-dependent manner. The protein expression and the mRNA semiquantitative expression of HDAC1 were enhanced obviously (P 0.05), but the expressions were down-regulated with the increase of the concentrations. Conclusion TSA can inhibit the proliferation of K562 cells and induce their apoptosis remarkably. One of their important mechanisms probably is that it can inhibit the expression of HDAC1.