Effects of baicalin on NCI-H292 cell apoptosis and acute lung injury induced by lipopolysaccharide

To explore the effects of baicalin on apoptosis of lung epithelial cell and acute lung injury induced by lipopolysaccharide(LPS).Apoptotic rates of lung epithelial cells induced by hydrogen peroxide were measured by Hoechst staining and flow cytometry.LPS was used to establish acute lung injury model of mice by intraperitoneal injection to observe the effects of baicalin on acute lung injury.ICR mice were randomly divided into 4groups:blank control,LPS,LPS+baicalin(150mg/(kg·d))and LPS+ baicalin(300mg/(kg·d)).Mice were administered intragastrically with distilled water or baicalin once a day for 3days.One hour after intragastrical treatment on the third day,LPS(20 mg/kg)was injected intraperitoneally except blank control group. Twelve hours after establishing the animal model,the histological changes of lungs were observed,myeloperoxidase(MPO)activity in lung homogenate was measured,and cytosolic phospholipase A2(cPLA2)and phosphorylated cytosolic phospholipase A2(phosphor-cPLA2)in lung tissues were detected by Western blotting.The results showed baicalin(60and 120μg/L)decreased the apoptosis of NCI-H292cells induced by H2O2,which was measured by Hoechst staining.Compared with the model group,baicalin concentration dependently suppressed the apoptosis of NCI-H292cells measured by flow cytometry.Twelve hours after LPS challenge,Histological studied showed that there were congestion,edema and the infiltrate of inflammatory cells in lung tissues in LPS group,and MPO activity and the levels of cPLA2in lung homogenate were highly increased.Instead lung injury was significantly alleviated in two dose baicalin +LPS groups.Baicalin has significant anti-apoptotic effects and could relieve LPS-induced acute lung injury by diminishing the contents of MPO and cPLA2both in vitro and in vivo.