Mechanisms of platelet-derived growth factor increasing activity of matrix metalloproteinase-2 in vascular smooth muscle cell

【Objective】 To investigate the expression of membrane type-1 matrix metalloproteinase(MT1-MMP) and tissue inhibitor of metalloproteinase-2(TIMP-2) in vascular smooth muscle cells(VSMCs) treated with platelet-derived growth factor(PDGF).Furthermore to explore the synergetic effects of MT1-MMP and TIMP-2 on matrix metalloproteinase-2(MMP-2) activity.【Methods】 After treatment with PDGF 0 min,20 min and 6 hours,MMP-2 activity was assayed by gel zymography.Meanwhile the expression of MT1-MMP and TIMP-2 were screened by using Chip and confirmed by RT-PCR.【Results】 The gel zymography showed that the activity of MMP-2 was significantly improved by PDGF.The expression of MT1-MMP was increased 1.86-fold and 2.93-fold for 20 min and 6 hrs treatment by PDGF compared with 0 min,respectively.And likewise the mRNA expressions of TIMP-2 were 1.35-fold and 1.82-fold higher in cultured with PDGF at 20 min and 6 hrs than 0min,respectively.【Conclusion】 PDGF significantly improved the expression of MT1-MMP and TIMP-2 in VSMCs and MMP-2 activity was enhanced by the coordination of MT1-MMP and TIMP-2.